We herein describe an ultrasensitive RNase H assay by utilizing CRISPR/Cas12a collateral cleavage activity. Based on this unique design principle, the RNase H activity was successfully determined down to 0.00024 U mL−1, which is quite superior to those of alternative approaches.
H. Kim, S. Lee, J. Lee and H. G. Park, Chem. Commun., 2022, 58, 2654 DOI: 10.1039/D1CC06026K
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