Determination of IC50 values of anticancer drugs on cells by D2O – single cell Raman spectroscopy

Wanxue Lv; Boqiang Fu; Manli Li; Yu Kang; Shouli Bai; Chao Lu

doi:10.1039/D1CC06857A

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Determination of IC₅₀ values of anticancer drugs on cells by D₂O – single cell Raman spectroscopy†

Wanxue Lv,^ab Boqiang Fu,

*^b Manli Li,^b Yu Kang,^a Shouli Bai^a and Chao Lu

*^a

Author affiliations

* Corresponding authors

^a State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China
E-mail: luchao@mail.buct.edu.cn

^b Center for Advanced Measurement Science (Institute of Life Science Metrology), National Institute of Metrology China, Beijing 100029, China
E-mail: fubq@nim.ac.cn

Abstract

A simple, sensitive and repeatable D₂O-single cell Raman spectroscopy method is developed to quantify the inhibitory activity of anticancer drugs on cancer cell metabolism. The IC₅₀ values obtained from A549 cells incubated with cisplatin and taxol are comparable with results of CCK-8 and ATP luminescent cell viability assays.

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Article information

DOI: https://doi.org/10.1039/D1CC06857A
Article type: Communication
Submitted: 06 Dec 2021
Accepted: 12 Jan 2022
First published: 17 Jan 2022

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Chem. Commun., 2022,58, 2355-2358

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Determination of IC₅₀ values of anticancer drugs on cells by D₂O – single cell Raman spectroscopy

W. Lv, B. Fu, M. Li, Y. Kang, S. Bai and C. Lu, Chem. Commun., 2022, 58, 2355 DOI: 10.1039/D1CC06857A

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Chemical Communications