Issue 28, 2022

A simple and rapid method to assay SARS-CoV-2 RNA based on a primer exchange reaction

Abstract

A simple method is proposed in this work for the detection of SARS-CoV-2 RNA based on a primer exchange reaction (PER). By ingeniously integrating the PER cascade and CRISPR/cas12a system, this method can achieve convenient detection of the target RNA in 40 min and distinguish a single-base mutation from the target sequence, demonstrating its superior analytical performance.

Graphical abstract: A simple and rapid method to assay SARS-CoV-2 RNA based on a primer exchange reaction

Supplementary files

Article information

Article type
Communication
Submitted
25 Jan 2022
Accepted
11 Mar 2022
First published
11 Mar 2022

Chem. Commun., 2022,58, 4484-4487

A simple and rapid method to assay SARS-CoV-2 RNA based on a primer exchange reaction

D. Li, C. Duan, W. Cheng, Y. gong, Y. Yao, X. Wang, Z. Wang and Y. Xiang, Chem. Commun., 2022, 58, 4484 DOI: 10.1039/D2CC00488G

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