Issue 39, 2022

Genetic encoding of a nonhydrolyzable phosphotyrosine analog in mammalian cells

Abstract

Protein tyrosine phosphorylation plays a critical role in signal transduction. We report the genetic incorporation of a phosphotyrosine (pTyr) analog, p-carboxymethyl-L-phenylalanine (CMF), into proteins in mammalian cells. This nonhydrolyzable pTyr analog can facilitate biological studies by removing complications caused by the dynamic interconversion between the phosphorylated and non-phosphorylated isoforms of a protein.

Graphical abstract: Genetic encoding of a nonhydrolyzable phosphotyrosine analog in mammalian cells

Supplementary files

Article information

Article type
Communication
Submitted
21 Mar 2022
Accepted
19 Apr 2022
First published
19 Apr 2022

Chem. Commun., 2022,58, 5897-5900

Author version available

Genetic encoding of a nonhydrolyzable phosphotyrosine analog in mammalian cells

X. He, B. Ma, Y. Chen, J. Guo and W. Niu, Chem. Commun., 2022, 58, 5897 DOI: 10.1039/D2CC01578A

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