Modelling the adsorption of phospholipid vesicles to a silicon dioxide surface using Langmuir kinetics

Abstract

Supported Lipid Bilayers (SLBs) are model biological membranes that have been developed to study the interactions between biomolecules in a cell membrane. Though forming SLBs is relatively easy, their formation mechanism remains a topic of debate. When buffered solutions containing phosphatidylcholine vesicles are flowed over a silicon dioxide (SiO₂) surface they adsorb intact to the surface to form a Supported Vesicle Layer (SVL) if the pH of the buffer is above 9. We have run experiments with buffers with a pH at or above 9 to study the kinetics of the adsorption of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) vesicles to an SiO₂ surface, which is the first step in the formation of an SLB. We used a quartz crystal microbalance (QCM) to monitor the real-time changes in the mass of the SVL as it formed from solutions with different lipid concentrations. Increases in the maximum frequency change with increasing lipid concentration indicated that both adsorption and desorption of DOPC vesicles were occurring, and that an equilibrium was established between the DOPC vesicles in the SVL and in the bulk solution. From the data acquired we were able to determine that the equilibrium constant for the adsorption and desorption of DOPC vesicles was 18 ± 1. The data was fitted to a Langmuir adsorption model from which the rate constants for the adsorption and desorption of DOPC vesicles were determined to be k_a = (0.0107 ± 0.0004) mL mg⁻¹ s⁻¹ and k_d = (5.8 ± 0.3) × 10⁻⁴ s⁻¹. The best fit to the experimental data was achieved if a parameter (α = (0.035 ± 0.003) s⁻¹) was used to account for the time taken for the lipid concentration to reach its steady state value in the flow cell used in the experiments.