Issue 3, 2022

Excess serum Na level in rats administered with high doses of (−)-epigallocatechin gallate-casein nanoparticles prepared with sodium caseinate

Abstract

(−)-Epigallocatechin gallate (EGCG)-incorporated casein nanoparticles benefit from excellent antioxidant, anti-inflammatory and anti-cancer activities due to their synergistic efficiency, but few studies have evaluated their safety. In this study, the EGCG-casein nanoparticles (EGCG-NPs) formulated using caseinate by ultrasonic treatment were evaluated for their subacute toxicity. The subacute toxicity test of EGCG-NPs through 28-day oral administration in rats did not exhibit adverse effect, with a no-observed-adverse-effect level (NOAEL) of at least 5.0 g per kg body weight (BW) per day, which was equivalent to 500 mg per kg BW EGCG per day. However, the serum Na level in females and males treated with 10.0 g per kg BW EGCG-NPs increased significantly as compared to the control rats (P < 0.05). Similar indications appeared in rats treated with 10.0 g per kg BW pure casein nanoparticles without EGCG, which indicated that high doses of caseinate nanoparticles result in an excess serum Na level. Therefore, we should consider the safety of the nanoparticle formulation of caseinate when it is used as a loading nutrient and a functional substance in foods.

Graphical abstract: Excess serum Na level in rats administered with high doses of (−)-epigallocatechin gallate-casein nanoparticles prepared with sodium caseinate

Supplementary files

Article information

Article type
Paper
Submitted
01 Sep 2021
Accepted
03 Nov 2021
First published
14 Jan 2022

Food Funct., 2022,13, 1291-1298

Excess serum Na level in rats administered with high doses of (−)-epigallocatechin gallate-casein nanoparticles prepared with sodium caseinate

Y. Zhang, S. Han, Y. Wang, H. Zhang, C. Yu, D. Qin, Q. Du and P. Jin, Food Funct., 2022, 13, 1291 DOI: 10.1039/D1FO02890A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements