A novel magnetophoretic-based device for magnetometry and separation of single magnetic particles and magnetized cells

Abstract

The use of magnetic micro- and nanoparticles in medicine and biology is expanding. One important example is the transport of magnetic microparticles and magnetized cells in lab-on-a-chip systems. The magnetic susceptibility of the particles is a key factor in determining their response to the externally applied magnetic field. Typically, to measure this parameter, their magnetophoretic mobility is studied. However, the particle tracking system for accurately determining the traveled distance in a certain time may be too complicated. Here, we introduce a lithographically fabricated chip composed of an array of thin magnetic micro-disks for evaluating the magnetic susceptibility of numerous individual magnetic particles simultaneously. The proposed novel magnetometer works based on the phase change in the trajectory of microparticles circulating around the disks in a rotating in-plane magnetic field. We explain that the easily detectable transition between the “phase-locked” and the “phase-slipping” regimes and the frequency at which it happens are appropriate parameters for measuring the magnetic susceptibility of the magnetic particles at the single-particle level. We show that this high-throughput (i.e., ∼ten thousand particles on a 1 cm² area) single-particle magnetometry method has various crucial applications, including i) magnetic characterization of magnetic beads as well as magnetically labeled living cells, ii) determining the magnetization rate of the cells taking up magnetic nanoparticles with respect to time, iii) evaluating the rate of degradation of magnetic nanoparticles in cells over time, iv) detecting the number of target cells in a sample, and v) separating particles based on their size and magnetic susceptibility.