Issue 10, 2022

Cilo-seq: highly sensitive cell-in-library-out single-cell transcriptome sequencing with digital microfluidics

Abstract

Single-cell RNA sequencing (scRNA-seq) plays a critical role in revealing genetic expression patterns at the single-cell level for cell type identification and rare transcript detection. Although there have been great advances in scRNA-seq methodologies, existing technologies still suffer from complexity and high cost, and an integrated platform for complete library construction is still lacking. Herein we describe Cilo-seq for high-performance scRNA-seq library construction in a single device with programmed and addressable droplet handling based on digital microfluidics. The platform is simultaneously accessible for convenient single-cell isolation, efficient nucleic acid amplification, low-loss nucleic acid purification and high-quality library preparation by leveraging specific interface design, tiny reaction volume, auxiliary magnetic field control and accurate droplet control. With a closed hydrophobic interface, the platform further reduces nucleic acid loss and exogenous background interference. Cilo-seq provides excellent detection sensitivity (1.4-fold improvement over tube-based methods), accuracy (R = 0.98) and cost efficiency (10-fold decrease in cost compared to tube-based methods), and holds great promise for studies of single-cell RNA biology.

Graphical abstract: Cilo-seq: highly sensitive cell-in-library-out single-cell transcriptome sequencing with digital microfluidics

Supplementary files

Article information

Article type
Paper
Submitted
21 Feb 2022
Accepted
11 Apr 2022
First published
11 Apr 2022

Lab Chip, 2022,22, 1971-1979

Cilo-seq: highly sensitive cell-in-library-out single-cell transcriptome sequencing with digital microfluidics

Q. Zhang, X. Xu, L. Lin, J. Yang, X. Na, X. Chen, L. Wu, J. Song and C. Yang, Lab Chip, 2022, 22, 1971 DOI: 10.1039/D2LC00167E

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements