Issue 4, 2022

Nanohybrid biosensor based on mussel-inspired electro-cross-linking of tannic acid capped gold nanoparticles and enzymes

Abstract

Complementary tools to classical analytical methods, enzymatic biosensors are widely applied in medical diagnosis due to their high sensitivity, potential selectivity, and their possibility of miniaturization/automation. Among the different protocols of enzyme immobilization, the covalent binding and cross-linking of enzymes ensure the great stability of the developed biosensor. Obtained manually by drop-casting using a specific cross-linker, this immobilization process is not suitable for the specific functionalization of a single electrode out of a microelectrode array. In the present work, we developed a nanohybrid enzymatic biosensor with high sensitivity by a mussel-inspired electro-cross-linking process using a cheap and abundant natural molecule (tannic acid, TA), gold salt, and native enzymes. Based on the use of a cheap natural compound and gold salt, this electro-cross-linking process based on catechol/amine reaction (i) is versatile, likely to be applied on any kind of enzymes, (ii) does not require the synthesis of a specific cross-linker, (ii) gives enzymatic biosensors with high and very stable sensitivity over two weeks upon storage at room temperature and (iv) is temporally and spatially controlled, allowing the specific functionalization of a single electrode out of a microelectrode array. Besides the development of microbiosensors, this process can also be used for the design of enzymatic biofuel cells.

Graphical abstract: Nanohybrid biosensor based on mussel-inspired electro-cross-linking of tannic acid capped gold nanoparticles and enzymes

Supplementary files

Article information

Article type
Paper
Submitted
16 Dec 2021
Accepted
24 Jan 2022
First published
25 Jan 2022
This article is Open Access
Creative Commons BY-NC license

Mater. Adv., 2022,3, 2222-2233

Nanohybrid biosensor based on mussel-inspired electro-cross-linking of tannic acid capped gold nanoparticles and enzymes

R. Savin, N. Benzaamia, C. Njel, S. Pronkin, C. Blanck, M. Schmutz and F. Boulmedais, Mater. Adv., 2022, 3, 2222 DOI: 10.1039/D1MA01193F

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements