Issue 8, 2022, Issue in Progress

Facile synthesis of peanut-like Sn-doped silica nano-adsorbent for affinity separation of proteins

Abstract

A peanut-like hollow silica (denoted as p-l-hSiO2) adsorbent is prepared in a facile method, which is composed of several silica nanospheres and has an average diameter of 22 nm, with thickness of 5 nm. Its Brunauer–Emmett–Teller (BET) surface area, pore volume and pore size are 258.9 m2 g−1, 1.56 cm3 g−1 and 3.9 nm, respectively. Then the afforded p-l-hSiO2/GSH adsorbent is applied to purify glutathione S-transferases-tagged (denoted as GST-tagged) proteins. It is found that the p-l-hSiO2 adsorbent exhibits a specific adsorption, a high binding capacity (6.80 mg g−1), good recycling performance and high recovery (90.1%) to the target proteins, showing promising potential for the affinity separation of GST-tagged proteins.

Graphical abstract: Facile synthesis of peanut-like Sn-doped silica nano-adsorbent for affinity separation of proteins

Article information

Article type
Paper
Submitted
15 Nov 2021
Accepted
25 Jan 2022
First published
08 Feb 2022
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2022,12, 4697-4702

Facile synthesis of peanut-like Sn-doped silica nano-adsorbent for affinity separation of proteins

M. Gao, Q. Liu, Y. Xue, B. Li, X. Liu, Z. Shi, N. Liu and X. Zou, RSC Adv., 2022, 12, 4697 DOI: 10.1039/D1RA08362G

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