A novel metal–organic framework of Ba–hemin with enhanced cascade activity for sensitive glucose detection

Abstract

Early glucose detection is important in both healthy people and diabetic patients. Glucose biosensing based on glucose oxidase (GOX) is a common method. However, native proteins are mostly membrane impermeable and are prone to degradation in complex sample environments. Herein, we report a facile one-step biomineralization method by simply mixing aqueous solutions of hemin and barium nitrate with glucose oxidase (GOX) to form Ba–hemin@GOX composites. Glucose (Glu) is introduced through self-driven sampling to trigger the GOX-catalysed production of hydrogen peroxide, which could help the subsequent 3,3′,5,5′-tetramethylbenzidine (TMB) oxidation reaction catalysed by Ba–hemin to yield the blue-coloured product. The sensor exhibited a detection limit as low as 3.08 μM. The operability and accuracy of the Ba–hemin@GOX biosensor were confirmed by the quantitative determination of glucose in real samples, such as tap water, serum and drinks. Moreover, the Ba–hemin@GOX-based colorimetric biosensor showed good selectivity, storage stability and recoverability. The experimental results reveal that a GOX activity of more than 90% was still maintained even after being incubated at 60 °C for 30 minutes, and Ba–hemin@GOX could be reused for glucose detection at least six times. Even after 30 days of storage, the relative activity was still more than 90%. Overall, the developed Ba–hemin@GOX biosensor provides a valuable and general platform for applications in colorimetric biosensing and medical diagnostics.