Issue 31, 2022
From the journal:

RSC Advances

Rapid and highly specific detection of site-specific 5-hydroxymethylcytosine based on peroxotungstate oxidation and mismatch ligation-based LAMP†

Zhenhao Zhang,ab   Tong He,ab   Yan Qi,a   Yuxuan Dai,a   Kejing Lao*a  and  Xingchun Gou ORCID logo *a  

* Corresponding authors

a Shaanxi Key Laboratory of Brain Disorders, Shaanxi Provincial Key Laboratory of Infection and Immunity Diseases, Institute of Basic and Translational Medicine, Xi'an Medical University, No. 1 Xin Wang Road, Xi'an 710021, China
E-mail: gouxingchun@189.cn
Tel: +86 29 86177603

b School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, Shaanxi Province, P. R. China

Abstract

We have developed a rapid and specific method for site-specific 5-hydroxymethylcytosine (5hmC) quantification at single-base resolution. This bisulfite-free method integrates the peroxotungstate oxidization with the mismatched probe-assisted ligation to guarantee the specificity. Moreover, the high-efficiency LAMP also makes the proposed method suitable for the detection of low-content samples.

Graphical abstract: Rapid and highly specific detection of site-specific 5-hydroxymethylcytosine based on peroxotungstate oxidation and mismatch ligation-based LAMP

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Article information

DOI
https://doi.org/10.1039/D2RA03310K
Article type
Paper
Submitted
26 May 2022
Accepted
21 Jun 2022
First published
07 Jul 2022
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2022,12, 19885-19889

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Rapid and highly specific detection of site-specific 5-hydroxymethylcytosine based on peroxotungstate oxidation and mismatch ligation-based LAMP

Z. Zhang, T. He, Y. Qi, Y. Dai, K. Lao and X. Gou, RSC Adv., 2022, 12, 19885 DOI: 10.1039/D2RA03310K

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