Synthesis and characterization of rod-like amino acids/nanohydroxyapatite composites to inhibit osteosarcoma

Abstract

In this study, rod-like hydroxyapatite (HA) with uniform morphology and controllable particle size modified by doping with two different amino acids (alanine and threonine) was synthesized by a microwave hydrothermal method. The physical and chemical properties of the composites were tested by utilizing X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), general thermogravimetric analysis (TG) and scanning electron microscopy (SEM). The SEM and XRD results show that the presence of amino acids (especially threonine) can significantly reduce the aspect ratio and crystallinity of hydroxyapatite. Pure hydroxyapatite and modified hydroxyapatite doped with two different proportions of amino acids were cultured with mouse osteoblasts (MC3T3-E1) for 1, 3 and 5 days, respectively, nanohydroxyapatite modified by threonine has better biocompatibility compared with pure hydroxyapatite. The amino acid-modified hydroxyapatite samples were co-cultured with osteosarcoma cells (MG63) for 1, 4 and 7 days, respectively, and showed better inhibitory effects on osteosarcoma cells. The nanohydroxyapatite doped with amino acids could be used as a potential drug that promotes bone repair and inhibits the growth of osteosarcoma cells.