Volume 1, 2022

Multi-coloured fluorescent sensing toolbox for selective detection of nitroxyl in vitro and ex vivo

Abstract

Methods for the endogenous detection of nitroxyl (azanone; HNO), the reduced and protonated derivative of nitric oxide (NO), are required to define its cardiovascular function and its key role in chronic pain. This study reports the design, synthesis and biological evaluation of 3 super-bright, highly sensitive, specific and non-cytotoxic arylphosphine-based fluorescent nitroxyl sensors that enable the detection of endogenous nitroxyl in vitro and ex vivo. The presence of endogenous nitroxyl is observed in the murine microglial cell line (BV2), primary human coronary artery endothelial cells (HCAECs) and rat cardiomyocyte cell line (H9C2) by spectroscopy and/or confocal microscopy following the addition of relevant stimulants. Furthermore, the presence of endogenous nitroxyl is detected in rat blood samples. The novel sensor 3 (rhodol 4 on a 2-(diphenylphosphanyl)benzoate backbone) is the most sensitive in detecting endogenous nitroxyl following stimulation, in the presence of biological media such as HBSS and DMEM. This then provides a validated tool for detecting nitroxyl in biological systems.

Graphical abstract: Multi-coloured fluorescent sensing toolbox for selective detection of nitroxyl in vitro and ex vivo

Supplementary files

Article information

Article type
Paper
Submitted
11 Jan 2022
Accepted
30 Jan 2022
First published
03 Feb 2022
This article is Open Access
Creative Commons BY-NC license

Sens. Diagn., 2022,1, 280-293

Multi-coloured fluorescent sensing toolbox for selective detection of nitroxyl in vitro and ex vivo

V. Staikopoulos, X. Zhang, B. P. Pullen, P. Reineck, A. K. Vidanapathirana, S. M. Lee, J. Liu, C. Bursill, M. R. Hutchinson and A. D. Abell, Sens. Diagn., 2022, 1, 280 DOI: 10.1039/D2SD00006G

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