Manganese dioxide nanosheet-containing reactors as antioxidant support for neuroblastoma cells

Abstract

Supporting mammalian cells against reactive oxygen species such as hydrogen peroxide (H₂O₂) is essential. Bottom-up synthetic biology aims to integrate designed artificial units with mammalian cells. Here, we used manganese dioxide nanosheets (MnO₂-NSs) as catalytically active entities that have superoxide dismutase-like and catalase-like activities. The integration of these MnO₂-NSs into 7 μm reactors was able to assist SH-SY5Y neuroblastoma cells when stressed with H₂O₂. Complementary, Janus-shaped 800 nm reactors with one hemisphere coated with MnO₂-NSs showed directed locomotion in cell media with top speeds up to 50 μm s⁻¹ when exposed to 300 mM H₂O₂ as a fuel, while reactors homogeneously coated with MnO₂-NSs were not able to outperform Brownian motion. These Janus-shaped reactors were able to remove H₂O₂ from the media, protecting cells cultured in the proximity. This effort advanced the use of bottom-up synthetic biology concepts in neuroscience.