Preparation and characterization of novel lithium magnesium phosphate bioceramic scaffolds facilitating bone generation

Abstract

Both magnesium and lithium are able to stimulate osteogenic and angiogenic activities. In this study, lithium magnesium phosphate (Li_0.5Mg_2.75(PO₄)₂, Li₁Mg_2.5(PO₄)₂ and Li₂Mg₂(PO₄)₂) biomaterials were synthesized by a solid-state reaction method, and their bioceramic blocks and scaffolds were fabricated by compression molding and 3D printing, respectively. The results indicated that the lithium magnesium phosphates consisted of the Mg₃(PO₄)₂ phase and/or LiMgPO₄ phase. Compared with the lithium-free Mg₃(PO₄)₂ bioceramics, the lithium magnesium phosphate bioceramics showed a lower porosity and consequently a higher compressive strength, and stimulated in vitro cellular proliferation, osteogenic differentiation and proangiogenic activity. In vivo results manifested that the Li₂Mg₂(PO₄)₂ bioceramic scaffolds efficiently promoted bone regeneration of critical-size calvarial defects in rats. Benefiting from the high compressive strength and capacity of stimulating osteogenesis and angiogenesis, the Li₂Mg₂(PO₄)₂ bioceramic scaffolds are considered promising for efficiently repairing the bone defects.