Issue 36, 2022

Macrocycle molecule-based cryoprotectants for ice recrystallization inhibition and cell cryopreservation

Abstract

Cyclodextrin-based cryoprotectants were developed. α-TMCD, which can be easily put into large-scale production, showed enhanced cell viabilities of 19.97 ± 0.78%, 13.93 ± 4.46% and 19.10 ± 0.95% against GES-1, hucMSCs and A549 cells. Moreover, the viable cells observed by light microscope imaging showed that the enhanced hucMSC cell number percentage of α-TMCD was 103.2%. An α-TMCD-DMSO-based CPA exhibited an enhanced cryoprotective effect by a mechanism of DMSO-enhanced cell penetrating effect and α-TMCD-DMSO synergistically enhanced IMA ability. α-TMCD exhibited potential for the discovery of macrocycle-molecule-based cryoprotectants.

Graphical abstract: Macrocycle molecule-based cryoprotectants for ice recrystallization inhibition and cell cryopreservation

Supplementary files

Article information

Article type
Communication
Submitted
22 May 2022
Accepted
10 Aug 2022
First published
11 Aug 2022

J. Mater. Chem. B, 2022,10, 6922-6927

Macrocycle molecule-based cryoprotectants for ice recrystallization inhibition and cell cryopreservation

B. Hu, G. Li, G. Ai, M. Zhang, S. Su, X. He, X. Li and K. Wang, J. Mater. Chem. B, 2022, 10, 6922 DOI: 10.1039/D2TB01083F

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