Development of a gold nanoparticle-based lateral-flow strip for the detection of cannabidiol in functional beverages

Abstract

Cannabidiol (CBD), the most predominant cannabinoid, may cause addiction and liver damage. In this study, we synthesized a CBD hapten containing the carboxyl group of the spacer arm by derivatizing CBD. A highly specific and sensitive monoclonal antibody (mAb) was prepared with a half-maximal inhibitory concentration of 2.03 ng mL⁻¹ and a limit of detection of 0.32 ng mL⁻¹. MAb was highly specific for CBD. We developed an immunochromatographic assay (ICA) for the detection of CBD in functional beverages with a visible limit of detection of 100 ng mL⁻¹ and a cut-off of 1000 ng mL⁻¹. Compared with HPLC, ICA is more efficient and accurate and can be used for the rapid on-site detection of CBD in samples.