Issue 12, 2023

Rapid determination of serum amyloid A using an upconversion luminescent lateral flow immunochromatographic strip

Abstract

The early diagnosis and real-time prognosis of cardiovascular diseases (CVDs) at the bedside are important. However, real-time detection of myocardial infarction involves the use of large-scale instrumentation and long test times. Herein, a simple, rapid and sensitive lateral flow immunochromatographic strip (LFIS) based on Yb/Er co-doped NaYF4 upconversion nanoparticles (UCNPs) was demonstrated for use in the detection of myocardial infarction. First, through heavy Yb/Er doping and an inert NaYF4 shell coating on the nanoparticles, the surface-related luminescence quenching effect of UCNPs was eliminated to enhance the upconversion luminescence. Second, through uniform coating of a SiO2 layer on the UCNPs, the biological affinity was improved to couple UCNPs and antibody proteins. Finally, through modification and activation with a specific antibody protein (serum amyloid A (SAA)), the UCNPs exhibited intense upconversion luminescence and high specificity when applied as a lateral flow immunochromatographic strip (LFIS). The developed UC-LFIS was highly sensitive (0.1 μg mL−1) and specific for detecting SAA in only 10 μL of serum. The UC-LFIS holds great potential for the early diagnosis and prognosis of CVDs.

Graphical abstract: Rapid determination of serum amyloid A using an upconversion luminescent lateral flow immunochromatographic strip

Supplementary files

Article information

Article type
Paper
Submitted
29 Mar 2023
Accepted
11 May 2023
First published
13 May 2023

Analyst, 2023,148, 2717-2724

Rapid determination of serum amyloid A using an upconversion luminescent lateral flow immunochromatographic strip

X. Sun, X. Dai, S. Ling, W. Dong, D. Chen, M. Li, X. Qiao, Z. Wang, X. Fan and G. Qian, Analyst, 2023, 148, 2717 DOI: 10.1039/D3AN00482A

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