Issue 23, 2023

A simple electrochemical strategy for the detection of the cancer marker CA19-9 by signal amplification using copper organic framework nanocomposite

Abstract

In this work, label-free electrochemical immunosensing of the cancer biomarker carbohydrate antigen 19-9 (CA19-9) is reported using [Fe(CN)6]3−/4− as a signal probe and a copper organic framework (Cu-BTC) nanocomposite for the amplification of the signal. The immunosensor was fabricated by the following process. First, the Cu-BTC nanomaterial with a larger surface area and good biocompatibility was synthesized to improve the dispersion of gold nanoparticles (Au NPs). Then, nitrogen-doped graphene (N-GR) was combined with Cu-BTC to form the nanocomposite. The synthesized Cu-BTC@N-GR@AuNPs@CS nanocomposite was employed to modify the surface of the immunosensor to accelerate the electron transfer rate and improve the immobilization amount of CA19-9 antibodies (Ab). Various techniques, including TEM, SEM and XPS were used to characterize Cu-BTC and nanocomposites. Differential pulse voltammetry (DPV) was used to measure the electrochemical response of the immunosensor in [Fe(CN)6]3−/4−. The signal intensity of the immunosensor was linearly changed upon increasing the concentration of CA19-9 antigen from 10 μU mL−1 to 100 U mL−1, and a detection limit of 4.2 μU mL−1 was achieved. Furthermore, the immunosensor showed good stability, reproducibility and specificity, indicating its potential application in clinical analysis.

Graphical abstract: A simple electrochemical strategy for the detection of the cancer marker CA19-9 by signal amplification using copper organic framework nanocomposite

Supplementary files

Article information

Article type
Paper
Submitted
02 Sep 2023
Accepted
30 Sep 2023
First published
06 Oct 2023

Analyst, 2023,148, 5905-5914

A simple electrochemical strategy for the detection of the cancer marker CA19-9 by signal amplification using copper organic framework nanocomposite

T. Zhang, Y. Ma and Y. Zhang, Analyst, 2023, 148, 5905 DOI: 10.1039/D3AN01511D

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