Screening of a short chain antimicrobial peptide-FWKFK and its application in wound healing

Abstract

As a kind of basic polypeptide with antibacterial properties, antimicrobial peptides play an important role in resisting the invasion of foreign microorganisms. Antimicrobial peptides have a wide range of antimicrobial activities against bacteria, fungi, viruses and other microorganisms. They are active against traditional antibiotic-resistant strains and do not easily cause bacterial resistance. In this study, we synthesized an antibacterial peptide library by a Fmoc solid phase synthesis method, and screened the peptide chain FWKFK by modified cell membrane chromatography. The minimum inhibitory concentration of FWKFK against E. coli and S. aureus was 200 μg mL⁻¹ and 250 μg mL⁻¹, respectively, and FWKFK also had inhibitory effects on P. aeruginosa, B. subtilis and S. epidermidis. Its biocompatibility and therapeutic effect on mouse wounds were then tested. The results showed that the survival rate of normal cells after FWKFK treatment was more than 95%, the hemolysis rate of red blood cells was as low as 6%, and it had a significant effect on wound healing in mice.