Issue 11, 2023
From the journal:

RSC Chemical Biology

A strategy for orthogonal deubiquitination using a bump-and-hole approach†

Takumi Suzuki,a   Yuki Utsugi,a   Satoshi Yamanaka,b   Hirotaka Takahashi,b   Yusuke Sato,cd   Tatsuya Sawasakib  and  Yusaku Miyamae ORCID logo *e  

* Corresponding authors

a Master's/Doctoral Program in Life Science Innovation, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan

b Division of Cell-Free Science, Proteo-Science Center, Ehime University, Matsuyama, Ehime, Japan

c Center for Research on Green Sustainable Chemistry, Tottori University, Tottori 680-8552, Japan

d Department of Chemistry and Biotechnology, Graduate School of Engineering, Tottori University, Tottori 680-8552, Japan

e Institute of Life and Environmental Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan
E-mail: miyamae.yusaku.fw@u.tsukuba.ac.jp

Abstract

We have successfully applied a bump-and-hole approach to establish orthogonal deubiquitination in which a ubiquitin substrate variant is specifically targeted by an engineered deubiquitinating enzyme (DUB). This makes it possibe to selectively observe and measure a single type of DUB activity in living cells.

Graphical abstract: A strategy for orthogonal deubiquitination using a bump-and-hole approach

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Article information

DOI
https://doi.org/10.1039/D3CB00095H
Article type
Communication
Submitted
19 Jun 2023
Accepted
11 Sep 2023
First published
02 Oct 2023
This article is Open Access
Creative Commons BY license

RSC Chem. Biol., 2023,4, 879-883

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A strategy for orthogonal deubiquitination using a bump-and-hole approach

T. Suzuki, Y. Utsugi, S. Yamanaka, H. Takahashi, Y. Sato, T. Sawasaki and Y. Miyamae, RSC Chem. Biol., 2023, 4, 879 DOI: 10.1039/D3CB00095H

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