Issue 77, 2023

Topological capture of mRNA for silencing gene expression

Abstract

We describe herein topological mRNA capture using branched oligodeoxynucleotides (ODNs) with multiple reactive functional groups. These fragmented ODNs efficiently formed topological complexes on template mRNA in vitro. In cell-based experiments targeting AcGFP mRNA, the bifurcated reactive ODNs showed a much larger gene silencing effect than the corresponding natural antisense ODN.

Graphical abstract: Topological capture of mRNA for silencing gene expression

Supplementary files

Article information

Article type
Communication
Submitted
16 Nov 2022
Accepted
23 Aug 2023
First published
04 Sep 2023

Chem. Commun., 2023,59, 11564-11567

Author version available

Topological capture of mRNA for silencing gene expression

F. Lyu, T. Tomita, N. Abe, H. Hiraoka, F. Hashiya, Y. Nakashima, S. Kajihara, F. Tomoike, Z. Shu, K. Onizuka, Y. Kimura and H. Abe, Chem. Commun., 2023, 59, 11564 DOI: 10.1039/D2CC06189A

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