Topological capture of mRNA for silencing gene expression

Fangjie Lyu; Takashi Tomita; Naoko Abe; Haruka Hiraoka; Fumitaka Hashiya; Yuko Nakashima; Shiryu Kajihara; Fumiaki Tomoike; Zhaoma Shu; Kazumitsu Onizuka; Yasuaki Kimura; Hiroshi Abe

doi:10.1039/D2CC06189A

Topological capture of mRNA for silencing gene expression†

Fangjie Lyu,^a Takashi Tomita,^a Naoko Abe,

^a Haruka Hiraoka,

^a Fumitaka Hashiya,

^a Yuko Nakashima,

^a Shiryu Kajihara,^a Fumiaki Tomoike,

^b Zhaoma Shu,^a Kazumitsu Onizuka,

^c Yasuaki Kimura

*^a and Hiroshi Abe

*^abde

Author affiliations

* Corresponding authors

^a Department of Chemistry, Graduate School of Science, Nagoya University, Furo, Chikusa, Nagoya 464-8602, Japan
E-mail: h-abe@chem.nagoya-u.ac.jp

^b Research Center for Materials Science, Nagoya University, Furo, Chikusa, Nagoya 464-8602, Japan

^c Institute of Multidisciplinary Research for Advanced Materials, Tohoku University 2-1-1 Katahira, Aoba-ku, Sendai, Miyagi 980-8577, Japan

^d CREST, Japan Science and Technology Agency, 7, Gobancho, Chiyoda-ku, Tokyo 102-0076, Japan

^e Institute for Glyco-core Research (iGCORE), Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8601, Japan

Abstract

We describe herein topological mRNA capture using branched oligodeoxynucleotides (ODNs) with multiple reactive functional groups. These fragmented ODNs efficiently formed topological complexes on template mRNA in vitro. In cell-based experiments targeting AcGFP mRNA, the bifurcated reactive ODNs showed a much larger gene silencing effect than the corresponding natural antisense ODN.

Chemical Communications

Topological capture of mRNA for silencing gene expression†

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