Reversible protein patterning on model membranes is important to reproduce spatiotemporal protein dynamics in vitro. An engineered version of iLID, disiLID, with a disordered domain as a membrane tether improves the recruitment of Nano under blue light and the reversibility in the dark, which enables protein patterning on membranes with higher spatiotemporal precision.
D. Di Iorio, J. Bergmann, S. L. Higashi, A. Hoffmann and S. V. Wegner, Chem. Commun., 2023, 59, 4380 DOI: 10.1039/D3CC00709J
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