Issue 27, 2023
From the journal:

Chemical Communications

DNA labelling in live cells via visible light-induced [2+2] photocycloaddition†

Rita T. Michenfelder,ab   Laura Delafresnaye, ORCID logo b   Vinh X. Truong, ORCID logo *bc   Christopher Barner-Kowollik ORCID logo *bd  and  Hans-Achim Wagenknecht ORCID logo *a  

* Corresponding authors

a Institute of Organic Chemistry, Karlsruhe Institute of Technology (KIT), Fritz-Haber Weg 6, Karlsruhe 76131, Germany
E-mail: wagenknecht@kit.edu

b School of Chemistry and Physics, Centre for Materials Science, Queensland University of Technology (QUT), 2 George St, Brisbane QLD 4000, Australia
E-mail: christopher.barnerkowollik@qut.edu.au

c Institute of Materials Research and Engineering, Agency for Science, Technology and Research (A*STAR), 2 Fusionopolis Way, Singapore
E-mail: vinh_truong@imre.a-star.edu.sg

d Institute of Nanotechnology, Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, Eggenstein-Leopoldshafen 76344, Germany
E-mail: christopher.barner-kowollik@kit.edu

Abstract

We introduce a visible light-driven (λmax = 451 nm) photo-chemical strategy for labelling of DNA in living HeLa cells via the [2+2] cycloaddition of a styrylquinoxaline moiety, which we incorporate into both the DNA and the fluorescent label. Our methodology offers advanced opportunities for the mild remote labelling of DNA in water while avoiding UV light activation.

Graphical abstract: DNA labelling in live cells via visible light-induced [2+2] photocycloaddition

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Article information

DOI
https://doi.org/10.1039/D3CC00817G
Article type
Communication
Submitted
21 Feb 2023
Accepted
02 Mar 2023
First published
02 Mar 2023

Chem. Commun., 2023,59, 4012-4015

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DNA labelling in live cells via visible light-induced [2+2] photocycloaddition

R. T. Michenfelder, L. Delafresnaye, V. X. Truong, C. Barner-Kowollik and H. Wagenknecht, Chem. Commun., 2023, 59, 4012 DOI: 10.1039/D3CC00817G

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