A ribose-functionalized NAD+ with versatile activity for ADP-ribosylation

Elisa N. Stephens; Xiao-Nan Zhang; Albert T. Lam; Jiawei Li; Hua Pei; Stan G. Louie; Clay C. C. Wang; Yong Zhang

doi:10.1039/D3CC04343F

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A ribose-functionalized NAD⁺ with versatile activity for ADP-ribosylation†

Elisa N. Stephens,

‡^a Xiao-Nan Zhang,

‡^a Albert T. Lam,^a Jiawei Li,^a Hua Pei,^b Stan G. Louie,^bc Clay C. C. Wang

^ad and Yong Zhang

*^acde

Author affiliations

* Corresponding authors

^a Department of Pharmacology and Pharmaceutical Sciences, Alfred E. Mann School of Pharmacy and Pharmaceutical Sciences, University of Southern California, Los Angeles, CA 90089, USA
E-mail: yongz@usc.edu

^b Titus Family Department of Clinical Pharmacy, Alfred E. Mann School of Pharmacy and Pharmaceutical Sciences, University of Southern California, Los Angeles, CA 90089, USA

^c Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, CA 90089, USA

^d Department of Chemistry, Dornsife College of Letters, Arts and Sciences, University of Southern California, Los Angeles, CA 90089, USA

^e Research Center for Liver Diseases, University of Southern California, Los Angeles, CA 90089, USA

Abstract

An NAD⁺ featuring an adenosyl 4′-azido functions as a general substrate for poly-ADP-ribose polymerases. Its derived mono- and poly-ADP-ribosylated proteins can be adequately recognized by distinct ADP-ribosylation-specific readers. This molecule represents the first ribose-functionalized NAD⁺ with versatile activities across different ADP-ribosyltransferases and provides insight into developing new probes for ADP-ribosylation.

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Article information

DOI: https://doi.org/10.1039/D3CC04343F
Article type: Communication
Submitted: 01 Sep 2023
Accepted: 29 Oct 2023
First published: 03 Nov 2023

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Chem. Commun., 2023,59, 13843-13846

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A ribose-functionalized NAD⁺ with versatile activity for ADP-ribosylation

E. N. Stephens, X. Zhang, A. T. Lam, J. Li, H. Pei, S. G. Louie, C. C. C. Wang and Y. Zhang, Chem. Commun., 2023, 59, 13843 DOI: 10.1039/D3CC04343F

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Chemical Communications