Issue 98, 2023

Artificial nucleotide codons for enzymatic DNA synthesis

Abstract

Herein, we report the high-yielding solid-phase synthesis of unmodified and chemically modified trinucleotide triphosphates (dN3TPs). These synthetic codons can be used for enzymatic DNA synthesis provided their scaffold is stabilized with phosphorothioate units. Enzymatic synthesis with three rather than one letter nucleotides will be useful to produce xenonucleic acids (XNAs) and for in vitro selection of modified functional nucleic acids.

Graphical abstract: Artificial nucleotide codons for enzymatic DNA synthesis

Supplementary files

Article information

Article type
Communication
Submitted
09 Oct 2023
Accepted
14 Nov 2023
First published
15 Nov 2023

Chem. Commun., 2023,59, 14547-14550

Artificial nucleotide codons for enzymatic DNA synthesis

N. Sabat, A. Stämpfli, M. Flamme, S. Hanlon, S. Bisagni, F. Sladojevich, K. Püntener and M. Hollenstein, Chem. Commun., 2023, 59, 14547 DOI: 10.1039/D3CC04933G

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