Ameliorating effects of Hericium erinaceus polysaccharides on intestinal barrier injury in immunocompromised mice induced by cyclophosphamide

Abstract

Hericium erinaceus is a kind of large fungus with rich nutrition and its polysaccharides exhibit various biological activities. In recent years, widespread interest has been focused on maintaining or improving intestinal health through the consumption of edible fungi. Studies have shown that hypoimmunity can damage the intestinal barrier, which in turn seriously affects human health. The aim of this work was to investigate the ameliorative effects of Hericium erinaceus polysaccharides (HEPs) on intestinal barrier damage in cyclophosphamide (CTX)-induced immunocompromised mice. The results showed that the HEP effectively increased the levels of total antioxidant capacity (T-AOC), glutathione peroxidase (GSH-PX), and total superoxide dismutase (T-SOD), and decreased malondialdehyde (MDA) content in the liver tissues of mice. In addition, the HEP restored the immune organ index, increased the serum levels of IL-2 and IgA, augmented the mRNA expression levels of intestinal Muc2, Reg3γ, occludin and ZO-1, and reduced intestinal permeability in mice. It was further confirmed by an immunofluorescence assay that the HEP enhanced the expression level of intestinal tight junction proteins to protect the intestinal mucosal barrier. These results suggested that the HEP could reduce intestinal permeability and enhance intestinal immune functions by increasing antioxidant capacity, tight junction proteins and immune-related factors in CTX-induced mice. In conclusion, the HEP effectively ameliorated CTX-induced intestinal barrier damage in immunocompromised mice, which provides a new application direction for the HEP as a natural immunopotentiator with antioxidant function.