Issue 20, 2023

In vivo potential of polymeric N-acryloyl-glycine nanoparticles with anti-inflammatory activities for wound healing

Abstract

An optimal wound-healing process requires highly coordinated and regulated interactions between the immune and biological systems since long-term wounds are severe clinical issues which make patients vulnerable to potentially fatal microbial infections. Therefore, it is essential to develop new treatments and therapeutic strategies. We aimed to develop a poly(N-acryloyl glycine) nanoparticle (PNAG NP) based formulation, which is effective to accelerate skin restoration. PNAG NPs ca. 35 nm in size (in dia.) have been synthesised through the mini-emulsion radical polymerisation technique and formulated with an ointment base for the establishment of wound healing application. Biocompatible, hemocompatible, proliferative and migration enhancer PNAG NPs were formulated as a PNAG nanoformulation, which showed a ∼31% higher wound healing efficiency compared to the control group of samples in the in vivo assessment. Periodic evaluation of TNF-α and IL6 for the treatment group shows the change in their levels from 4.10 ± 3.18 to 5.17 ± 3.27 pg mL−1 and 11.93 ± 2.34 pg mL−1 to 10.33 ± 3.31 pg mL−1, respectively, consistent with the reduction in inflammation. The increased level of IGF-1 from 25.41 ± 13.56 ng mL−1 to 64.22 ± 16.93 ng mL−1 in the PNAG test group could be correlated with the increase in angiogenesis followed by an increase in the wound healing efficiency. This study showed that PNAG NPs could execute rapid coordination between cell proliferation and migration, anti-inflammatory actions, and simultaneous regeneration of skin tissues without the use of any of the external synergistic factors like drugs, genes or cells. Thus, the intriguing results showed that the PNAG NPs could address the existing wound healing challenges and can serve as a potential material for regenerative purposes.

Graphical abstract: In vivo potential of polymeric N-acryloyl-glycine nanoparticles with anti-inflammatory activities for wound healing

Supplementary files

Article information

Article type
Paper
Submitted
07 Jul 2023
Accepted
21 Aug 2023
First published
24 Aug 2023
This article is Open Access
Creative Commons BY-NC license

Mater. Adv., 2023,4, 4718-4731

In vivo potential of polymeric N-acryloyl-glycine nanoparticles with anti-inflammatory activities for wound healing

P. S. Gupta, K. Wasnik, G. Singh, S. Patra, D. Pareek, D. D. Yadav, M. S. Tomar, S. Maiti, M. Singh and P. Paik, Mater. Adv., 2023, 4, 4718 DOI: 10.1039/D3MA00378G

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements