Issue 12, 2023

Freezing-assisted reverse microemulsion synthesis of hollow mesoporous silica encapsulated glucose oxidase

Abstract

Bio-activity conservation of biomolecules after immobilizing in nanomaterials has been a tough challenge, especially in biomedical applications. Herein, we developed a novel freezing-assisted reverse microemulsion method to encapsulate water-soluble enzymes in hollow mesoporous silica nanospheres (HMSNs) for maximumly maintaining enzyme bio-activity. Firstly, a low-temperature and ethanol-free synthesis was carried out, and glucose oxidase (GOx) as a model enzyme was encapsulated into HMSNs to obtain GOx@HMSNs. The TEM and SEM images showed a uniform sphere morphology of GOx@HMSNs with a large hollow cavity for GOx loading. The element mapping, FTIR spectrum and TGA curve further confirmed the effective physical encapsulation of GOx in HMSNs, which can maintain stable bio-activity as verified by the enzyme activity assay. In addition to the facile synthesis for avoiding enzyme-denaturation, the N2 adsorption–desorption curves further demonstrated that small mesopores on HMSNs for blocking enzyme leakage may be another important factor for conserving GOx activity. Finally, GOx@HMSNs as a biosensor significantly possessed a wider linear range of 0.06 mM to 2.61 mM with a low detection limit of 0.01 mM, indicating high sensitivity. Therefore, our synthesis method provides a promising approach for biomolecule immobilization with potent bio-activity and biosensor applications with superior sensitivity.

Graphical abstract: Freezing-assisted reverse microemulsion synthesis of hollow mesoporous silica encapsulated glucose oxidase

Supplementary files

Article information

Article type
Paper
Submitted
06 Jul 2022
Accepted
19 Jan 2023
First published
09 Feb 2023

New J. Chem., 2023,47, 5773-5779

Freezing-assisted reverse microemulsion synthesis of hollow mesoporous silica encapsulated glucose oxidase

J. Cao, F. Shi, L. Chen, X. Xu, Z. Chen, Z. Yang and X. Jiang, New J. Chem., 2023, 47, 5773 DOI: 10.1039/D2NJ03324K

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