Issue 43, 2023

A wash-free fluorescent probe with a large Stokes shift for the identification of NAFL through tracing the change of lipid droplets

Abstract

As one of the important organelles in cells, lipid droplets (LDs) are involved in various physiological processes, especially affecting the occurrence and progression of non-alcoholic fatty liver (NAFL). Therefore, it is of great significance to develop LD-specific probes with excellent biocompatibility, deep penetration and bright fluorescence. Herein, a fluorescent probe LD-HWZ was designed and synthesized based on triphenylamine and the dicyanoisophorone group. It is found that probe LD-HWZ has a large Stokes shift (Δλ = 160 nm in DMSO) and exhibits bright fluorescence in a lipid environment. In addition, biological experiments showed that LD-HWZ can localize in lipid droplets, which can be used to detect the dynamic changes of LDs. Importantly, LD-HWZ has been successfully used to discriminate NAFL tissues from normal livers. The excellent properties of probe LD-HWZ in this work are expected to shed new light on the design of lipid droplet probes for the study of fatty liver diagnosis.

Graphical abstract: A wash-free fluorescent probe with a large Stokes shift for the identification of NAFL through tracing the change of lipid droplets

Supplementary files

Article information

Article type
Paper
Submitted
03 Sep 2023
Accepted
20 Oct 2023
First published
20 Oct 2023

Org. Biomol. Chem., 2023,21, 8767-8771

A wash-free fluorescent probe with a large Stokes shift for the identification of NAFL through tracing the change of lipid droplets

J. Yang, Z. Wang, Y. Deng, C. Zhang, X. Shen, J. He, L. Hu and H. Wang, Org. Biomol. Chem., 2023, 21, 8767 DOI: 10.1039/D3OB01410J

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