Issue 5, 2023

Gold nanoparticle-based lateral flow immunoassay for the rapid detection of flutriafol residues in food

Abstract

As a commonly used crop fungicide with proven adverse effects against various organisms, the excessive use of flutriafol (FTF) has resulted in accumulation of a large amount of residues in crops. In this study, a novel hapten was designed by introducing a long interval arm on its benzene ring allowing sufficient exposure of the FTF active site, for the production of a highly sensitive and specific monoclonal antibody with a half-inhibitory concentration of 1.77 ng mL−1. Furthermore, for the first time, a gold nanoparticle-based lateral flow immunoassay (LFIA) was developed for the quantitative detection of FTF in foods with a visual limit of detection in rice, tomatoes, and grapes of 0.5 mg kg−1, 0.2 mg kg−1 and 0.5 mg kg−1, respectively, and a cutoff value of 4 mg kg−1, and a calculated limit of detection of 0.045 mg kg−1, 0.035 mg kg−1 and 0.060 mg kg−1, respectively. The recoveries in spiked samples ranged from 90.2% to 110.1% and these results indicated that the gold nanoparticle-based LFIA developed in this study for the rapid detection of FTF in food is a reliable on-site detection method.

Graphical abstract: Gold nanoparticle-based lateral flow immunoassay for the rapid detection of flutriafol residues in food

Supplementary files

Article information

Article type
Research Article
Submitted
29 Nov 2022
Accepted
19 Jan 2023
First published
19 Jan 2023

Mater. Chem. Front., 2023,7, 955-963

Gold nanoparticle-based lateral flow immunoassay for the rapid detection of flutriafol residues in food

Y. Liu, L. Liu, X. Xu, C. Xu and L. Xu, Mater. Chem. Front., 2023, 7, 955 DOI: 10.1039/D2QM01231F

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