Issue 38, 2023, Issue in Progress
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RSC Advances

Metal-enhanced fluorescence through conventional Ag-polyethylene glycol nanoparticles for cellular imaging

Chih-Jung Chen,abc   Chun-Yen Wu,d   Chi-Wei Wu,d   Ching-Wen Chang,d   Tsung-Tao Huang,e   Ming-Hua Shiao,e   Chu-Kuei Lin,f   Yu-Chun Chen*d  and  Yung-Sheng Lin ORCID logo *d  

* Corresponding authors

a Department of Pathology and Laboratory Medicine, Taichung Veterans General Hospital, Taichung 407219, Taiwan

b School of Medicine, Chung Shan Medical University, Taichung 402306, Taiwan

c Department of Post-Baccalaureate Medicine, College of Medicine, National Chung Hsing University, Taichung 402202, Taiwan

d Department of Chemical Engineering, National United University, Miaoli 360302, Taiwan
E-mail: joycechen@nuu.edu.tw, linys@nuu.edu.tw

e Taiwan Instrument Research Institute, National Applied Research Laboratories, Hsinchu 302058, Taiwan

f PoloWang Bio. Inc, New Taipei City 234011, Taiwan

Abstract

A novel application of conventional Ag nanoparticles (NPs) for metal-enhanced fluorescence (MEF) in cellular imaging is proposed. Different molecular weights of polyethylene glycol (PEG) were tested to determine a suitable spacer on Ag NPs for MEF, and NPs comprising Ag with PEG with a molecular weight of 6000 g (Ag-PEG6k), when present in fluorescein solution, were discovered to cause a 2-fold quantum yield enhancement. For fluorescence imaging of mesenchymal stem cells stained by Alexa Fluor 488, the enhancement factor increased with the Ag-PEG6k NP concentration but decreased with the Alexa Fluor 488 concentration. At 243 parts per billion Ag-PEG6k NPs and 625 parts per million Alexa Fluor 488, the enhancement factor reached its greatest value of over 4.

Graphical abstract: Metal-enhanced fluorescence through conventional Ag-polyethylene glycol nanoparticles for cellular imaging

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Article information

DOI
https://doi.org/10.1039/D3RA02277C
Article type
Paper
Submitted
06 Apr 2023
Accepted
21 Aug 2023
First published
05 Sep 2023
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2023,13, 26545-26549

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Metal-enhanced fluorescence through conventional Ag-polyethylene glycol nanoparticles for cellular imaging

C. Chen, C. Wu, C. Wu, C. Chang, T. Huang, M. Shiao, C. Lin, Y. Chen and Y. Lin, RSC Adv., 2023, 13, 26545 DOI: 10.1039/D3RA02277C

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