Morphologies, mechanical and in vitro behaviors of DLP-based 3D printed HA scaffolds with different structural configurations

Abstract

In the field of bone engineering, porous ceramic scaffolds are in great demand for repairing bone defects. In this study, hydroxyapatite (HA) ceramic scaffolds with three different structural configurations, including the body-centered cubic (BCC), the face-centered cubic (FCC), and the triply periodic minimal surface (TPMS), were fabricated through digital light processing (DLP) based 3D printing technologies. The effects of the structural configurations on the morphologies and mechanical properties of the DLP-based 3D printed HA scaffolds were characterized. Furthermore, in vitro evaluations, including in vitro cytocompatibility, bone alkaline phosphatase (ALP) activity assay, and protein expression, were conducted to assess HA scaffold behavior. Finally, we evaluated the effects of structural configurations from these aspects and selected the most suitable structure of HA scaffold for bone repair.