Non-enzymatic amperometric glucose sensing on CuO/mesoporous TiO2 modified glassy carbon electrode

Abstract

The present study illustrates the fabrication of a glucose sensing electrode based upon binary composite of copper oxide and mesoporous titanium dioxide on glassy carbon (CuO/TiO₂/GCE). The X-ray diffraction, scanning electron microscopy and energy dispersive X-ray analysis evidently showed the phase pure monoclinic CuO nanoparticles and anatase TiO₂. N₂ adsorption–desorption analysis verified the mesoporosity in TiO₂ with specific surface area greater than 105 m² g⁻¹. Electrochemical impedance spectroscopic analysis proved the remarkable decrease in the charge transfer resistance and facilitation of electron transfer process on the fabricated electrode. The optimum weight ratio of CuO to TiO₂ was 1 : 1, and the optimum potential was 0.6 V vs. saturated calomel electrode. The chronoamperometric measurements displayed a detection limit of 1.9 μM, and sensitivities of 186.67 μA mM⁻¹ cm⁻² and 90.53 μA mM⁻¹ cm⁻² in two linear ranges of 0.05 to 5.2 mM and 5.2 to 20 mM, respectively. The amperometric analysis further showed good reproducibility, high specificity and outstanding stability of the modified electrode.