A highly selective Hg2+ colorimetric sensor and antimicrobial agent based on green synthesized silver nanoparticles using Equisetum diffusum extract

Abstract

Plasmonic nanoparticles such as Ag have gained great interest in the biomedical domain and chemical analysis due to their unique optical properties. Herein, we report a simple, cost-effective, and highly selective colorimetric sensor of mercury(II) based on E. diffusum (horsetail) extract-functionalized Ag nanoparticles (ED-AgNPs). The ED-AgNPs were synthesized by exploiting the coordination of Ag⁺ with the various functional groups of ED extract under sunlight exposure for only tens of seconds. ED-AgNPs (63 nm) were characterized using various techniques such as UV-vis, FTIR, DLS, SEM and EDX. FTIR spectra suggested the successful encapsulation of the AgNPs surface with ED extract and XRD confirmed its crystalline nature. This ED-AgNPs colorimetric sensor revealed remarkable selectivity towards Hg²⁺ in aqueous solution among other transition metal ions through a redox reaction mechanism. Besides, the sensor exhibited high sensitivity with rapid response and a detection limit of 70 nM. The sensor demonstrated feasibility for Hg(II) detection in spiked tap and river water samples. In addition, the synthesized ED-AgNPs revealed enhanced antimicrobial activity with higher efficacy against the Gram-positive bacterium (L. monocytogenes with an inhibition zone of 18 mm) than the Gram-negative bacterium (E. coli with an inhibition zone of 10 mm). The simplicity and adaptability of this colorimetric sensor render it a promising candidate for on-site and point-of-care detection of heavy metal ions in diverse conditions.