Issue 4, 2023

Dynamic mechanical analysis of suspended soft bodies via hydraulic force spectroscopy

Abstract

The rheological characterization of soft suspended bodies, such as cells, organoids, or synthetic microstructures, is particularly challenging, even with state-of-the-art methods (e.g. atomic force microscopy, AFM). Providing well-defined boundary conditions for modeling typically requires fixating the sample on a substrate, which is a delicate and time-consuming procedure. Moreover, it needs to be tuned for each chemistry and geometry. Here, we validate a novel technique, called hydraulic force spectroscopy (HFS), against AFM dynamic indentation taken as the gold standard. Combining experimental data with finite element modeling, we show that HFS gives results comparable to AFM microrheology over multiple decades, while obviating any sample preparation requirements.

Graphical abstract: Dynamic mechanical analysis of suspended soft bodies via hydraulic force spectroscopy

Supplementary files

Article information

Article type
Paper
Submitted
30 Aug 2022
Accepted
07 Nov 2022
First published
08 Nov 2022
This article is Open Access
Creative Commons BY-NC license

Soft Matter, 2023,19, 615-624

Dynamic mechanical analysis of suspended soft bodies via hydraulic force spectroscopy

M. Berardi, K. Gnanachandran, J. Jiang, K. Bielawski, C. W. Visser, M. Lekka and B. I. Akca, Soft Matter, 2023, 19, 615 DOI: 10.1039/D2SM01173E

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