Dehydration of biological membranes in a non-condensing environment

Abstract

The study of the dehydration process in a cell membrane allows a better understanding of how water is bound to it. While in prior studies, cell dehydration was commonly analyzed under osmotic stress conditions, in the present work, we focus on the dehydration driven by evaporation in a restricted condensing environment. Using a thermogravimetry method, we studied the dehydration of Escherichia coli through isothermal evaporation in the presence of a gas flux. To figure out the loss of mass in this situation, we first evaluated the dynamics of water evaporation of a suspension of multilamellar liposomes. We found that the evaporation of liposomal suspensions composed of individual lipids is constant, although slightly restricted by the presence of liposomes, while the evaporation of liposomal suspensions composed of a mixture of different lipids follows an exponential decay. This is explained considering that the internal pressure at the air–water interface is proportional to the amount of bound water. The evaporation of water from a biomass sample follows this latter behaviour.