Hybrid reduced graphene oxide nanosheets with negative magnetoresistance for the diagnosis of hypoglycemia

Abstract

Few glucometers are available to easily and quickly measure low blood glucose levels (≤4 mmol L⁻¹) from a small amount of blood samples. Here, a hybrid reduced graphene oxide (rGO)-based magnetoresistance (MR) sensor has been developed to monitor blood glucose levels to quickly detect hypoglycemia. Hybrid rGO nanosheets, incorporating Fe₅₀Co₅₀ nanoparticles onto rGO nanosheets, with an unusual large negative MR (−5.7%) at room temperature under a small magnetic field (9.5 kOe) have been successfully fabricated through a one-pot reaction. To quickly detect the low concentration of glucose in a small amount of blood (1 μL), a two-step process has been further developed by using the “sandwich” structural MR sensor. The results show that the higher the negative MR value of the sensor, the lower the concentration of glucose that can be detected. A linear relationship between the MR and the concentration of the spiked plasma glucose taken from streptozotocin-induced diabetic rats can be found when the concentration of glucose is in the range of 0–6 mmol L⁻¹. The limit of detection (LOD) of this MR glucose sensor is 0.867 mmol L⁻¹. The accuracy of the rGO-based MR sensor is improved in measuring low concentration of plasma glucose as compared to that of a commercialized glucometer. Furthermore, the selectivity of the rGO-based MR sensor has been studied. The results demonstrate that the rGO-based MR sensor is a flexible and sensitive detection platform and specifically suitable for monitoring low concentrations of plasma glucose to prevent from hypoglycemia.