Issue 2, 2024

A 3D hanging spheroid-filter plate for high-throughput drug testing and CAR T cell cytotoxicity assay

Abstract

Tumour spheroids are widely used in immune cell cytotoxicity assays and anticancer drug testing, providing a physiologically relevant model replicating the tumour microenvironment. However, co-culture of immune and tumour cells complicates quantification of immune cell killing efficiency. We present a novel 3D hanging spheroid-filter plate that efficiently facilitates spheroid formation and separates unbound/dead cells during cytotoxicity assays. Optical imaging directly measures the cytotoxic effects of anti-cancer drugs on tumour spheroids, eliminating the need for live/dead fluorescent staining. This approach enables cost-effective evaluation of T-cell cytotoxicity with specific chimeric antigen receptors (CARs), enhancing immune cell-based assays and drug testing in three-dimensional tumour models.

Graphical abstract: A 3D hanging spheroid-filter plate for high-throughput drug testing and CAR T cell cytotoxicity assay

Supplementary files

Article information

Article type
Paper
Submitted
02 Nov 2023
Accepted
28 Nov 2023
First published
29 Nov 2023

Analyst, 2024,149, 475-481

A 3D hanging spheroid-filter plate for high-throughput drug testing and CAR T cell cytotoxicity assay

Z. Chen, S. Han, S. Kim, C. Lee, A. Sanny, A. H. Tan and S. Park, Analyst, 2024, 149, 475 DOI: 10.1039/D3AN01904G

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