Ag supraparticles with 3D hot spots to actively capture molecules for sensitive detection by surface enhanced Raman spectroscopy

Abstract

To achieve highly sensitive detection using surface-enhanced Raman spectroscopy (SERS), it is imperative to fabricate a substrate with a high density of hot spots and facilitate the entry of target molecules into these hot spot regions. However, steric hindrance arising from the presence of surfactants and ligands on the SERS substrate may impede the access of target molecules to the hot spots. Here, we fabricate non-close-packed three-dimensional (3D) supraparticles with high-density hot spots to actively capture molecules. The formation of 3D supraparticles is attributed to the minimization of free energy during the gradual contraction of the droplet. The numerous capillaries present in non-close-packed supraparticles induce the movement of target molecules into the hot spot region through capillary force along with the solution. The results demonstrate that the SERS enhancement effect of 3D supraparticles is at least one order of magnitude higher than that of multi-layered nanoparticle structures formed under natural drying conditions. In addition, the SERS performance of 3D supraparticles is evaluated with diverse target molecules, including antimicrobial agents and drugs. Hence, this work provides a new idea for the preparation of non-close-packed substrates for SERS sensitive detection.