Using FT-IR as a fast method to measure fatty acid soaps in in vivo and in vitro digests

Abstract

The objective of the current study was to develop a simple method to measure fatty acid soaps, making use of FT-IR, representative for the soap formation observed in clinical trials. Calcium soaps have a unique coordination which leads to a typical double-splitting of the antisymmetric and symmetric carboxylate peaks. Absorbance values of these carboxylate peaks were used together with the absorbance of the hydrocarbon –CH₂ antisymmetric and symmetric peaks to calculate the calcium soap absorbance. Based on the linear correlation between the calcium soap absorbance and the calcium soap concentration measured with GC-FID, a model was set-up and subsequently successfully validated to quantify calcium soap concentrations in faecal samples from clinical trials with this FT-IR method. With in vivo as well as in vitro digestion an inverse correlation between the long chain saturated fatty acid part of milk fat containing fat blends used for the infant formulas, and the formation of fatty acid soaps after digestion and defaecation could be observed. There is a clear link between the amount of long chain saturated fatty acids at the sn-1/3 position and their release as free fatty acid after lipolysis with the appearance of fatty acid soaps. These insights enable future development of fat blends for infant nutrition to optimize fatty acid soap formation and thereby gut discomfort in infants. These insights can be used to predict the soap formation capacity of a newly designed fat blend and thereby the improvement of infant nutrition products.