Development of a fluorous trapping reagent for rapid detection of electrophilic reactive metabolites

Abstract

A cysteine-based fluorous trapping reagent, Rf₈CYS, was developed. Rf₈CYS formed adducts with soft and hard electrophilic reactive metabolites. These fluorous-tagged adducts were purified via both fluorous solid-phase extraction and the direct injection method. The highly sensitive mass spectrometric detection of an unprecedented adduct of the ticlopidine metabolite was realized.