A native mass spectrometry approach to qualitatively elucidate interfacial epitopes of transient protein–protein interactions

Clinton G. L. Veale; Abir Chakraborty; Richwell Mhlanga; Fernando Albericio; Beatriz G. de la Torre; Adrienne L. Edkins; David J. Clarke

doi:10.1039/D4CC01251H

A native mass spectrometry approach to qualitatively elucidate interfacial epitopes of transient protein–protein interactions†

Clinton G. L. Veale,

*^a Abir Chakraborty,

^b Richwell Mhlanga,^b Fernando Albericio,

^c Beatriz G. de la Torre,

^d Adrienne L. Edkins^b and David J. Clarke

*^e

Author affiliations

* Corresponding authors

^a Department of Chemistry, University of Cape Town, Rondebosch, Cape Town 7701, South Africa
E-mail: clinton.veale@uct.ac.za

^b The Biomedical Biotechnology Research Unit (BioBRU), Department of Biochemistry and Microbiology, Rhodes University, Makhanda, South Africa

^c School of Chemistry and Physics, University of KwaZulu-Natal, Westville, South Africa

^d School of Laboratory Medicine and Medical Sciences, College of Health Sciences, University of KwaZulu-Natal, South Africa

^e EaStCHEM School of Chemistry, University of Edinburgh, Joseph Black Building, David Brewster Road, Edinburgh EH93FJ, UK
E-mail: dave.clarke@ed.ac.uk

Abstract

Native mass spectrometric analysis of TPR2A and GrpE with unpurified peptides derived from limited proteolysis of their respective PPI partners (HSP90 C-terminus and DnaK) facilitated efficient, qualitative identification of interfacial epitopes involved in transient PPI formation. Application of this approach can assist in elucidating interfaces of currently uncharacterised transient PPIs.

Supplementary files

Article information

DOI: https://doi.org/10.1039/D4CC01251H
Article type: Communication
Submitted: 18 Mar 2024
Accepted: 13 May 2024
First published: 13 May 2024
This article is Open Access

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Chem. Commun., 2024,60, 5844-5847

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A native mass spectrometry approach to qualitatively elucidate interfacial epitopes of transient protein–protein interactions

C. G. L. Veale, A. Chakraborty, R. Mhlanga, F. Albericio, B. G. de la Torre, A. L. Edkins and D. J. Clarke, Chem. Commun., 2024, 60, 5844 DOI: 10.1039/D4CC01251H

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Chemical Communications

A native mass spectrometry approach to qualitatively elucidate interfacial epitopes of transient protein–protein interactions†

Abstract

Supplementary files

Article information

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A native mass spectrometry approach to qualitatively elucidate interfacial epitopes of transient protein–protein interactions

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