Programmability of dual-color DNA-templated silver nanoclusters for modular design of FRET aptasensors toward multiplexed detection

Abstract

By exploiting the programmability of DNA, dual-color DNA-templated silver nanoclusters have been synthesized to serve as a label-free fluorescent probe with a G5-linker at the 3′ end. This advancement facilitates the modular design of universal FRET-based aptasensors using aptamers with a C5-linker at the 3′ end for multiplexed detection, making them easily switch their applications.