Issue 96, 2024

Laccase-catalyzed tyrosine click reaction with 1-methyl-4-arylurazole: rapid labeling on protein surfaces

Abstract

Our study demonstrates the exceptional efficiency of 1-methyl-4-arylurazole (MAUra) for tyrosine labeling, optimized with laccase under mild conditions, achieving a high efficiency (kcat/Km = 7.88 × 104 M−1 s−1) with minimal oxidative side reactions and selective labeling of highly exposed tyrosine sites on proteins.

Graphical abstract: Laccase-catalyzed tyrosine click reaction with 1-methyl-4-arylurazole: rapid labeling on protein surfaces

Supplementary files

Article information

Article type
Communication
Submitted
29 Jul 2024
Accepted
28 Oct 2024
First published
12 Nov 2024
This article is Open Access
Creative Commons BY license

Chem. Commun., 2024,60, 14208-14211

Laccase-catalyzed tyrosine click reaction with 1-methyl-4-arylurazole: rapid labeling on protein surfaces

K. Nakane, C. Fujimura, S. Miyano, Z. Liu, T. Niwa, H. Nishi, T. Kadonosono, H. Taguchi, S. Tomoshige, M. Ishikawa and S. Sato, Chem. Commun., 2024, 60, 14208 DOI: 10.1039/D4CC03802A

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