Installation of an organocatalyst into a protein scaffold creates an artificial Stetterase†
Eva
Klemencic, 
a
Richard C.
Brewster,
a
Evangelia
Notari,
ab
Amanda G.
Jarvis
*a
and
Dominic J.
Campopiano
*a
a
Richard C.
Brewster,
a
Evangelia
Notari,
ab
Amanda G.
Jarvis
*a
and
Dominic J.
Campopiano
*a
Abstract
Using a protein scaffold covalently functionalised with a thiamine-inspired N-heterocyclic carbene (NHC), we created an artificial Stetterase (ArtiSt) which catalyses a stereoselective, intramolecular Stetter reaction. We demonstrate that ArtiSt functions under ambient conditions with low catalyst loading. Furthermore, activity can be increased >20 fold by altering the protein scaffold.
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