A low iridium content greatly improves the peroxidase-like activity of noble metal nanozymes for sensitive colorimetric detection

Abstract

The enzyme-like activity of noble metal nanomaterials has been widely demonstrated. However, as an important noble metal, iridium (Ir) and its alloy nanomaterials have been less studied, particularly regarding the effect of Ir content on enzyme-like activity. Here, we demonstrated for the first time that a low Ir content can greatly improve the peroxidase-like activity of Pt-based nanozymes. When the weight percentage of Ir was 3.45% in trimetallic PtAuIr hollow nanorods (HNRs) and 2.86% in bimetallic PtIr HNRs, their specific activity increased by approximately 70% compared to their PtAu and Pt counterparts, respectively. However, a slightly higher percentage of Ir significantly diminished the enhancement effect on their specific activity. Density functional theory (DFT) calculations show that the rate-determining step (RDS) energy barrier of the nanozyme with low Ir content is lower than that of the nanozyme with slightly higher Ir content. Furthermore, we studied the kinetic properties of the PtAuIr nanozyme using TMB as the substrate. Its Michaelis–Menten constant (K_m) and V_max were 1.756 mM and 2.152 × 10⁻⁶ M s⁻¹, respectively. Additionally, a colorimetric detection platform based on the PtAuIr nanozyme was established and applied to detect o-phenylenediamine (OPD), with a detection limit as low as 0.076 μM. This study highlights the important role of the Ir content in Pt-based nanozymes and demonstrates that PtAuIr nanozymes have potential applications in environmental detection.