Issue 22, 2024

Photocatalytic acylation of lysine screened using a microfluidic-based chemical robotic system

Abstract

The application scope of various bioconjugation technologies has been expanded through the implementation of photoredox catalytic bioconjugation technology, establishing a novel biomolecular framework with exceptional residue selectivity. Herein, we report a facile acylation reaction utilizing a thioacid and a photoexcited riboflavin tetraacetate (RFTA) catalyst under visible light (450 nm). The optimal reaction conditions were obtained using a high-throughput microfluidic-based chemical robotic system and GPR model. We have successfully modified a range of proteins and antibodies and utilized the functional handle to attach diverse biological molecules, demonstrating the versatility and generality of our approach. In addition, this photoredox catalytic reaction was also successfully employed in peptide modification, protein labeling, and antibody conjugation.

Graphical abstract: Photocatalytic acylation of lysine screened using a microfluidic-based chemical robotic system

Supplementary files

Article information

Article type
Paper
Submitted
20 Jul 2024
Accepted
17 Sep 2024
First published
02 Oct 2024

Green Chem., 2024,26, 11238-11248

Photocatalytic acylation of lysine screened using a microfluidic-based chemical robotic system

Z. Hou, C. Wan, H. Jiang, Y. Wang, Y. Xing, J. Wang, Z. Liu, X. Guo, Y. An, W. Han, R. Wang, X. Zhang, F. Yin and Z. Li, Green Chem., 2024, 26, 11238 DOI: 10.1039/D4GC03562C

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