Glucose oxidase intercalation into polydopamine and ZIF-8 as a highly efficient and stable bio-catalyst for H2O2 production

Abstract

Glucose oxidase has great potential as a bio-catalyst for the determination of glucose and H₂O₂ concentration, but it is sensitive to environmental influences and has poor recoverability. To overcome this limitation, this study presents a promising approach, i.e., glucose oxidase interposed with dopamine and ZIF-8 to develop a highly efficient and stable bio-catalyst for H₂O₂ production while determining glucose concentrations. The successful synthesis of GOx@ZIF-8/PDA was verified by SEM, EDS, XRD, FTIR, XPS, and UV analyses; GOx@ZIF-8/PDA exhibited higher stability, better reproducibility, and excellent glucose assay potential compared with the free enzyme. After 7 days of storage, GOx@ZIF-8/PDA almost maintained its initial activity, and the catalytic activity remained at 92.15 ± 2.13% after five consecutive applications. This study provides a new strategy for the preparation of highly active and stable immobilized enzymes.